The analytical techniques routinely used in Biochemistry take a very important place in Biochemistry, learning them is not just a requirement but, absolutely essential. This is because, the understanding of the subject mainly comes from the strong basis of the experiments and techniques on which the theories are built. This course deals with various techniques their principles, experimental details etc., including general Laboratory techniques, separation techniques based on hydrodynamic properties, centrifugation (sub-cellular fractionation etc), and Chromatographic, and Electrophortic techniques , HPLC, Purification methods, ELISA etc. Application in biomedical research and some clinical diagnostic methods RIA, ELISA, PET etc., Moleular diagnostics ,PCR, etc., Microscopic techniques and clinical biochemistry.
COURSE LAYOUT Week 1
Aqueous solutions , Acids, Bases, buffers systems and pH meter,Colorimetry and UV-VIS absorption spectroscopy,Introduction to Hydrodynamic Techniques Week 2 Centrifugation: Principles & Methodology,Density Gradient centrifugation : Rate Zonal and isopycnic,Differential centrifugation for Sub-cellular Fractionation Week 3 Gel filtration: Principle, Methodology & Applications,Planar Chromatography: Principles and Applications Week 4 Ion-exchange chromatography,Affinity chromatography Week 5Gas chromatography,HPLC,Protein Estimation Techniques Week 6 Assessment-2,Light Microscopy and Confocal Microscopy,Fluorescence Microscopy : Application to live cell imaging Week 7IHC and IF,Flow cytometry: Theory and concept,Flow cytometry:Application in biology and medicine Week 8 Electron Microscopy, TEM and SEM,SDS-PAGE Week 9 Western Blotting Techniques,2D Electrophoresis and DIGE,Principles of Mass spectrometry Week 10 Clinical Proteomics,Methods to quantify and Integrity check of DNA Week 11 Methods to quantify and Integrity check of RNA,PCR and Real Time PCR,Blotting techniques for Nucleic acidsWeek 12 Sequencing techniques of nucleic acids,Introduction to Recombinant DNA Technology,Assessment-2 Week 13 Expression techniques of recombinant proteins using Bacteria and yeast,Expression techniques of recombinnat proteins by insects and mammalian cells,Introduction to Genomic Techniques Week 14 Techniques for studying Nucleic acid protein interactions,ELISA, RIA :Diagnostic applications Week 15 CLIA and its Diagnostic applications,PET and its Diagnostic applications,Single cell biology,Final Examination